PFKFB1

Available structures

Ortholog search: PDBe RCSB

List of PDB id codes
1K6M

Identifiers

Aliases

PFKFB1, F6PK, HL2K, PFRX, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1

External IDs

MGI: 107816 HomoloGene: 105654 GeneCards: PFKFB1

Gene ontology
Molecular function	• transferase activity • nucleotide binding • fructose-6-phosphate binding • kinase activity • kinase binding • catalytic activity • protein binding • identical protein binding • fructose-2,6-bisphosphate 2-phosphatase activity • hydrolase activity • ATP binding • 6-phosphofructo-2-kinase activity
Cellular component	• cytosol • 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase complex
Biological process	• gluconeogenesis • response to glucagon • glycolytic process • phosphorylation • canonical glycolysis • fructose metabolic process • response to glucocorticoid • carbohydrate phosphorylation • response to insulin • positive regulation of glucokinase activity • fructose 2,6-bisphosphate metabolic process • animal organ regeneration • response to starvation • metabolic process • response to cAMP • dephosphorylation
Sources:Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

Entrez


5207


18639

Ensembl


ENSG00000158571


ENSMUSG00000025271

UniProt


P16118


P70266

RefSeq (mRNA)


NM_001271804 NM_001271805 NM_002625


NM_008824

RefSeq (protein)


NP_001258733.1 NP_001258734.1 NP_002616.2


NP_032850.1

Location (UCSC)

Chr X: 54.93 – 55 Mb

Chr X: 150.59 – 150.64 Mb

PubMed search

^[1]

^[2]

Wikidata

View/Edit Human

View/Edit Mouse

6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 is an enzyme that in humans is encoded by the PFKFB1 gene.^[3]^[4]

This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis.^[4]

References

↑ "Human PubMed Reference:".
↑ "Mouse PubMed Reference:".
↑ Batra RS, Hatchwell E, Rider S, Brown R, Brown GK, Craig IW (Apr 1997). "Localization of human liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB1) within a YAC contig in Xp11.21". Genomics. 40 (2): 358–61. doi:10.1006/geno.1996.4530. PMID 9119406.
1 2 "Entrez Gene: PFKFB1 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1".

Lange AJ, Pilkis SJ (1990). "Sequence of human liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase". Nucleic Acids Res. 18 (12): 3652. doi:10.1093/nar/18.12.3652. PMC 331036. PMID 2163524.
Marker AJ, Colosia AD, Tauler A, et al. (1989). "Glucocorticoid regulation of hepatic 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene expression". J. Biol. Chem. 264 (12): 7000–4. PMID 2540168.
Algaier J, Uyeda K (1988). "Molecular cloning, sequence analysis, and expression of a human liver cDNA coding for fructose-6-P,2-kinase:fructose-2,6-bisphosphatase". Biochem. Biophys. Res. Commun. 153 (1): 328–33. doi:10.1016/S0006-291X(88)81226-9. PMID 2837207.
Lee YH, Li Y, Uyeda K, Hasemann CA (2003). "Tissue-specific structure/function differentiation of the liver isoform of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase". J. Biol. Chem. 278 (1): 523–30. doi:10.1074/jbc.M209105200. PMID 12379646.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Ross MT, Grafham DV, Coffey AJ, et al. (2005). "The DNA sequence of the human X chromosome". Nature. 434 (7031): 325–37. doi:10.1038/nature03440. PMC 2665286. PMID 15772651.
Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: Large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.

PDB gallery

1c7z: REGULATORY COMPLEX OF FRUCTOSE-2,6-BISPHOSPHATASE

1c80: REGULATORY COMPLEX OF FRUCTOSE-2,6-BISPHOSPHATASE

1c81: MICHAELIS COMPLEX OF FRUCTOSE-2,6-BISPHOSPHATASE

1fbt: THE BISPHOSPHATASE DOMAIN OF THE BIFUNCTIONAL RAT LIVER 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE

1k6m: Crystal Structure of Human Liver 6-Phosphofructo-2-Kinase/Fructose-2,6-Bisphosphatase

1tip: THE BISPHOSPHATASE DOMAIN OF THE BIFUNCTIONAL RAT LIVER 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE

Metabolism: carbohydrate metabolism: glycolysis/gluconeogenesis enzymes

Glycolysis

Gluconeogenesis only

to oxaloacetate:	Pyruvate carboxylase Phosphoenolpyruvate carboxykinase

from lactate (Cori cycle):	Lactate dehydrogenase

from alanine (Alanine cycle):	Alanine transaminase

from glycerol:	Glycerol kinase Glycerol dehydrogenase

Regulatory

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PFKFB1

References

Further reading