Prostaglandin-E2 9-reductase
| prostaglandin-E2 9-reductase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 1.1.1.189 | ||||||||
| CAS number | 42613-35-4 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / EGO | ||||||||
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In enzymology, a prostaglandin-E2 9-reductase (EC 1.1.1.189) is an enzyme that catalyzes the chemical reaction
- (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+ (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate + NADPH + H+
Thus, the two substrates of this enzyme are (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate and NADP+, whereas its 3 products are (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate, NADPH, and H+.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate:NADP+ 9-oxidoreductase. Other names in common use include PGE2-9-OR, reductase, 15-hydroxy-9-oxoprostaglandin, 9-keto-prostaglandin E2 reductase, 9-ketoprostaglandin reductase, PGE-9-ketoreductase, PGE2 9-oxoreductase, PGE2 reductase-9-ketoreductase, prostaglandin 9-ketoreductase, prostaglandin E 9-ketoreductase, and prostaglandin E2 reductase-9-oxoreductase. This enzyme participates in arachidonic acid metabolism.
Structural studies
As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 1Q13, 1Q5M, and 2PFG.
References
- Lee SC, Levine L (1975). "Purification and regulatory properties of chicken heart prostaglandin E 9-ketoreductase". J. Biol. Chem. 250 (12): 4549–55. doi:10.1016/S0016-6995(02)00041-4. PMID 166995.
- Schlegel W, Kruger S, Korte K (1984). "Purification of prostaglandin E2 reductase-9-oxoreductase from human decidua vera". FEBS Lett. 171 (1): 141–4. doi:10.1016/0014-5793(84)80475-5. PMID 6586494.
- Tai HH, Yuan B (1982). "Purification and assay of 9-hydroxyprostaglandin dehydrogenase from rat kidney". Methods Enzymol. 86: 113–7. doi:10.1016/0076-6879(82)86178-8. PMID 7132747.
- Watkins JD, Jarabak J (1985). "The effect of NaCl intake on 9-ketoprostaglandin reductase activity in the rabbit kidney". Prostaglandins. 30 (2): 335–49. doi:10.1016/0090-6980(85)90196-0. PMID 3901124.