Purnell equation
The Purnell equation is an equation used in analytical chemistry to calculate the resolution Rs between two peaks in a chromatogram:[1][2][3]
where
- Rs is the resolution between the two peaks
- N2 is the plate number of the second peak
- α is the separation factor between the two peaks
- k '2 is the retention factor of the second peak.
The higher the resolution, the better the separation.
References
- ↑ Miller, J. M., Chromatography: Concepts and Contrasts, second edition, John Wiley & Sons, Inc., Hoboken, NJ, USA, 2005.
- ↑ Dean, J. R. et al., Practical Skills in Chemistry, first edition, Pearson Education Ltd., Harlow, UK, 2002, p. 205–224.
- ↑ C. Legido-Quigley, HPLC theory
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