SF3A1
Splicing factor 3 subunit 1 is a protein that in humans is encoded by the SF3A1 gene.[3][4]
This gene encodes subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family; named for the SURP (also called SWAP or Suppressor-of-White-APricot) motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs. Alternative splicing results in multiple transcript variants encoding different isoforms.[4]
Interactions
SF3A1 has been shown to interact with SF3A3[5][6] and CDC5L.[7]
References
Further reading
- Chiara MD, Champion-Arnaud P, Buvoli M, et al. (1994). "Specific protein-protein interactions between the essential mammalian spliceosome-associated proteins SAP 61 and SAP 114.". Proc. Natl. Acad. Sci. U.S.A. 91 (14): 6403–7. doi:10.1073/pnas.91.14.6403. PMC 44210
. PMID 8022796.
- Rain JC; Tartakoff AM; Krämer A; Legrain P (1996). "Essential domains of the PRP21 splicing factor are implicated in the binding to PRP9 and PRP11 proteins and are conserved through evolution.". RNA. 2 (6): 535–50. PMC 1369393. PMID 8718683.
- Neubauer G, King A, Rappsilber J, et al. (1998). "Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex.". Nat. Genet. 20 (1): 46–50. doi:10.1038/1700. PMID 9731529.
- "Toward a complete human genome sequence.". Genome Res. 8 (11): 1097–108. 1999. doi:10.1101/gr.8.11.1097. PMID 9847074.
- Krämer A; Grüter P; Gröning K; Kastner B (1999). "Combined biochemical and electron microscopic analyses reveal the architecture of the mammalian U2 snRNP.". J. Cell Biol. 145 (7): 1355–68. doi:10.1083/jcb.145.7.1355. PMC 2133165. PMID 10385517.
- Dunham I, Shimizu N, Roe BA, et al. (1999). "The DNA sequence of human chromosome 22.". Nature. 402 (6761): 489–95. doi:10.1038/990031. PMID 10591208.
- Das R; Zhou Z; Reed R (2000). "Functional association of U2 snRNP with the ATP-independent spliceosomal complex E.". Mol. Cell. 5 (5): 779–87. doi:10.1016/S1097-2765(00)80318-4. PMID 10882114.
- Gunther M; Laithier M; Brison O (2000). "A set of proteins interacting with transcription factor Sp1 identified in a two-hybrid screening.". Mol. Cell. Biochem. 210 (1–2): 131–42. doi:10.1023/A:1007177623283. PMID 10976766.
- Ajuh P, Kuster B, Panov K, et al. (2001). "Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry.". EMBO J. 19 (23): 6569–81. doi:10.1093/emboj/19.23.6569. PMC 305846. PMID 11101529.
- Suzuki Y, Tsunoda T, Sese J, et al. (2001). "Identification and characterization of the potential promoter regions of 1031 kinds of human genes.". Genome Res. 11 (5): 677–84. doi:10.1101/gr.164001. PMC 311086. PMID 11337467.
- Will CL, Schneider C, MacMillan AM, et al. (2001). "A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site.". EMBO J. 20 (16): 4536–46. doi:10.1093/emboj/20.16.4536. PMC 125580. PMID 11500380.
- Nesic D; Krämer A (2001). "Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation.". Mol. Cell. Biol. 21 (19): 6406–17. doi:10.1128/MCB.21.19.6406-6417.2001. PMC 99788. PMID 11533230.
- Jurica MS, Licklider LJ, Gygi SR, et al. (2002). "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis.". RNA. 8 (4): 426–39. doi:10.1017/S1355838202021088. PMC 1370266. PMID 11991638.
- Will CL, Urlaub H, Achsel T, et al. (2002). "Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein.". EMBO J. 21 (18): 4978–88. doi:10.1093/emboj/cdf480. PMC 126279. PMID 12234937.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins.". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
- Nesic D; Tanackovic G; Krämer A (2005). "A role for Cajal bodies in the final steps of U2 snRNP biogenesis.". J. Cell. Sci. 117 (Pt 19): 4423–33. doi:10.1242/jcs.01308. PMID 15316075.
- Lin KT; Lu RM; Tarn WY (2004). "The WW domain-containing proteins interact with the early spliceosome and participate in pre-mRNA splicing in vivo.". Mol. Cell. Biol. 24 (20): 9176–85. doi:10.1128/MCB.24.20.9176-9185.2004. PMC 517884. PMID 15456888.
- Collins JE, Wright CL, Edwards CA, et al. (2005). "A genome annotation-driven approach to cloning the human ORFeome.". Genome Biol. 5 (10): R84. doi:10.1186/gb-2004-5-10-r84. PMC 545604. PMID 15461802.
External links
PDB gallery |
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| 1we7: Solution structure of Ubiquitin-like domain in SF3a120 |
| 1zkh: Solution structure of a human ubiquitin-like domain in SF3A1 |
| 2dt6: Solution structure of the first SURP domain of human splicing factor SF3a120 |
| 2dt7: Solution structure of the second SURP domain of human splicing factor SF3a120 in complex with a fragment of human splicing factor SF3a60 |
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