SPI1

SPI1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1PUE
Identifiers
Aliases	SPI1, OF, PU.1, SFPI1, SPI-1, SPI-A, Spi-1 proto-oncogene
External IDs	MGI: 98282 HomoloGene: 2346 GeneCards: SPI1
Gene ontology Molecular function • DNA binding • sequence-specific DNA binding • core promoter binding • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding • RNA polymerase II core promoter proximal region sequence-specific DNA binding • transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding • transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding • transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding • protein binding • RNA binding • RNA polymerase II transcription factor activity, sequence-specific DNA binding • RNA polymerase II core promoter sequence-specific DNA binding • transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding • RNA polymerase II transcription factor binding • RNA polymerase II distal enhancer sequence-specific DNA binding • transcription factor activity, sequence-specific DNA binding • NFAT protein binding Cellular component • nucleus • nuclear chromatin Biological process • myeloid leukocyte differentiation • regulation of transcription, DNA-templated • cellular response to ethanol • somatic stem cell population maintenance • lymphocyte differentiation • regulation of transcription from RNA polymerase II promoter • regulation of erythrocyte differentiation • myeloid dendritic cell differentiation • negative regulation of transcription from RNA polymerase II promoter • negative regulation of MHC class II biosynthetic process • transcription from RNA polymerase II promoter • lymphoid progenitor cell differentiation • transcription, DNA-templated • macrophage differentiation • anatomical structure regression • negative regulation of histone H4 acetylation • negative regulation of gene expression, epigenetic • vasculature development • granulocyte differentiation • apoptotic process involved in blood vessel morphogenesis • histone H3 acetylation • erythrocyte differentiation • hypermethylation of CpG island • positive regulation of pri-miRNA transcription from RNA polymerase II promoter • positive regulation of transcription from RNA polymerase II promoter • negative regulation of transcription, DNA-templated • positive regulation of transcription, DNA-templated Sources:Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	6688	20375
Ensembl	ENSG00000066336	ENSMUSG00000002111
UniProt	P17947	P17433
RefSeq (mRNA)	NM_001080547 NM_003120	NM_011355
RefSeq (protein)	NP_001074016.1 NP_003111.2	NP_035485.1
Location (UCSC)	Chr 11: 47.35 – 47.38 Mb	Chr 2: 91.08 – 91.12 Mb
PubMed search	[1]	[2]
Wikidata

Transcription factor PU.1 is a protein that in humans is encoded by the SPI1 gene.^[3]

Function

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development.[Citation needed] The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.^[4]

Interactions

SPI1 has been shown to interact with:

• FUS,^[5]
• GATA2,^[6]
• IRF4,^[7][8] and
• NONO.^[9]

See also

• Spi-1 (PU.1) 5' UTR regulatory element

References

Further reading

External links

• FactorBook PU.1

PDB gallery
1pue: PU.1 ETS DOMAIN-DNA COMPLEX