Lysine iron agar slant

The lysine iron agar slant or LIA slant test is used to distinguish bacteria which are able to decarboxylate lysine and/or produce hydrogen sulfide from those that cannot. This test is particularly useful for distinguishing different Gram-negative bacilli—especially among the Enterobacteriaceae.

Procedure

The LIA slant is inoculated by stabbing the agar to within 1/4 inch of the butt of the tube and streaking the slant. The slant is then incubated at 35°C for 18–24 hours. The results are scored as follows:

purple slant/yellow butt = LSI Negative
turbid, purple butt = LSI Positive
black precipitate = H₂S Positive

Growth media / agar plates

Selective media

Gram positive

Actinobacteria	Mycobacterium tuberculosis Löwenstein–Jensen medium Middlebrook 7H9 Broth Middlebrook 7H10 Agar Middlebrook 7H11 Agar Mycoplasma pneumoniae Eaton's agar

Firmicutes	Corynebacterium diphtheriae Hoyle's agar Enterococcus Bile esculin agar Lactobacillus MRS agar Lactococcus M17 agar Staphylococcus Mannitol salt agar

Gram negative

Alphaproteobacteria	Brucella Brucella agar Farrell's medium

Betaproteobacteria	Neisseria Thayer-Martin agar

Gammaproteobacteria	Bordetella Bordet-Gengou agar Enterobacteriaceae VRBD agar Haemophilus influenzae/Legionella pneumophila Buffered charcoal yeast extract agar Pseudomonas aeruginosa Cetrimide agar Salmonella XLT agar DCA agar Salmonella/Shigella XLD agar

Differential media

Fungal media

Nonselective media

Other/ungrouped media

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